Involvement of Stat1 in the Phagocytosis of M. avium
Figure 1
Time course of JAK/STAT1 signalling pathway activation after internalization of latex particles, heat-killed and live M. avium into macrophages. (a) Left: figure of western blot analysis of Stat1 and actin levels at 0, 24, 48, and 72 hours after uptake. The control (CTR) is represented by untreated macrophages; right: in the graph, we report the quantitative analysis of Stat1 normalized to the amount of actin and expressed as percentage of control value which is placed as 100%. (b) Left: figure of western blot analysis of p-tyr701Stat1 and actin levels at 0, 24, 48, and 72 hours after uptake. The control (CTR) is represented by untreated macrophages; right: in the graph, we report the quantitative analysis of p-tyr701Stat1 normalized to the amount of actin and expressed as percentage of control value which is placed as 100%. The results are the mean of five different experiments. Statistical analyses were performed with the Student’s t-test (Prism 4 Graph Pad software): (#) significant difference from control (CTR) .
