Involvement of Stat1 in the Phagocytosis of M. avium

Table 1

We correlate the Stat1/p-tyr₇₀₁Stat1 densitometric value with the entity of internalized material in the presence or absence of Fc mAb IV.3 as FcγR inhibitor. Stat1 and p-tyr₇₀₁Stat1 levels were normalized to the amount of actin and expressed as percentage of control value which was placed as 100%. The results are the mean of five different experiments. The control (CTR) for proteins’ expression levels were represented by untreated macrophages. The correlation between internalization of FcγR and Stat1/p-tyr Stat1 pathway activity was determined at 24 hours after phagocytosis.

	Stat 1 (percent versus CTR)	p-tyr701Stat1 (percent versus CTR)	Number bacteria/cell
M. avium (AB)	200 ± 13.0	247 ± 12.0	12.2 ± 3.3
M. avium (AB) + Fc mAb IV.3	100 ± 12.4	113 ± 9.0	11.3 ± 2.2
			(95% versus serum AB)
CTR^*	100	100	—
HK M. avium (AB)	148 ± 7.2	658 ± 29.3	13.2 ± 3.5
HK M. avium (AB) + Fc mAb IV.3	112 ± 11.0	116 ± 5.0	5.0 ± 2.0
			(35% versus serum AB)

^*CTR (100%): human macrophages not exposed.