Regulatory T Cells Accumulate in the Lung Allergic Inflammation and Efficiently Suppress T-Cell Proliferation but Not Th2 Cytokine Production

Figure 7

Lung Foxp3⁺ Treg cells from allergic mice suppress T-cell proliferation but not type 2 cytokine production. (a) IL-4 and (b) IL-5 levels in the supernatants of FACS-sorted lung CD4⁺Foxp3-GFP⁺ (Foxp3⁺) or CD4⁺Foxp3-GFP⁻ (Foxp3⁻) cells from Foxp3gfp.KI allergic mice alone or cocultured at different Foxp3⁻/Foxp3⁺ ratios. (c) Proliferation of Foxp3-GFP^- cells cocultured at different Foxp3⁻/Foxp3⁺ ratios was determined by flow cytometry. Sorted lung CD4⁺Foxp3-GFP^- were labeled with Dye eFluor-670 and the proliferation was determined by reduction of the fluorescence intensity of Dye. Representative FACS-histograms, which indicate the frequency of T-cell proliferation, are shown. Values represent the means ± SEM of triplicate wells. The results are representative of two experiments. Significant differences **𝑃<0.01, ***𝑃<0.001 are shown.