Identification of RD5-Encoded Mycobacterium tuberculosis Proteins As B-Cell Antigens Used for Serodiagnosis of Tuberculosis

Table 1

Primers and vectors used for cloning of RD5 genes.

Gene	Forward primer^a (5′–3′)	Reverse primer^a (5′–3′)	Restriction endonucleases used	Vectors	Annealing temperature (°C)	Amplicon size (bp)
Rv3117	CATACCATGGCACGCTGCGATG	CTTCTCGAGTCAGCTTCCCAAC	Nco I and Xho I	pET-32a	58	834
Rv3118	GGAATTCCATATGTGCTCTGGACCC	CTGCTCGAGGGTGATCTTGACGTCTAC	Nde I and Xho I	pET-21a	59	303
Rv3119	CATGCCATGGCCAATGTGGTAG	CTTCTCGAGTGGTCTATCGCCGAC	Nco1 and Xho I	pET-28b	57	444
Rv3120	CAAGGTACCATGAGTCCGTCTCCATCG	CCTAAGCTTCTACAGTGACCGTTGGGC	Kpn I and Hind III	pET-32a	63	603
Rv3121	GATATTCCATATGACAAGCACCTCG	CATAAGCTTCGTCGGCCAGGTAAC	Nde I and Hind III	pET-21a	58	1203

^aThe restriction sites used for cloning are underlined.