Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells
Figure 5
Adhesion molecules expressed on NK cells during mouse ontogeny. At the indicated time points, mononuclear cells were isolated from the liver and spleen and analysed by flow cytometry for the expression of adhesion molecules. NK cells (NK1.1+CD3−) were gated, and each marker was analysed by histogram. In each independent experiment, six to seven fetal mouse livers or spleens were put together to acquire enough cells to perform FACS analysis in one experiment, and three independent experiments were performed. In the other groups, there were three mice independently detected for one experiment, and three independent experiments were performed. The expression of CD11c (a) and CD73 (b) on liver NK cells is shown and compared with spleen NK cells during ontogeny. The mean fluorescence intensity (MFI) and percentage of each marker is shown as the mean ± SEM from three mice in each group. These are from a single experiment representative of three independent experiments. ***, **, * compared with the corresponding group.
