Development of Murine Hepatic NK Cells during Ontogeny: Comparison with Spleen NK Cells

Figure 6

Neonatal liver NK cells produced more IFN-gamma, perforin, and Granzyme B than 10-week-old liver NK cells after stimulated with Poly I: C in vitro. In each independent experiment, six to seven neonatal mouse livers/spleens were put together to acquire enough cells to perform FACS analysis. NK cells (NK1.1⁺CD3⁻) were gated, and then each marker was analyzed by histogram. Unstained controls are the grey. (a) Each percentage represents the net percentage (%) of cells in the appropriate quadrant. (b) The percentages of the IFN-gamma⁺ NK cell subset (%) were calculated for the total number of NK cells in the liver and spleen. Data are shown as mean ± SEM three mice in each group. ***𝑃<0.001, **𝑃<0.01, *𝑃<0.05 compared with the corresponding group.

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