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Figure 2: Images acquired by AKLIDES: typical C-ANCA on ethanol-fixed neutrophils (a) and formalin-fixed neutrophils (b); typical P-ANCA on ethanol-fixed neutrophils (c) and switch to C-ANCA on formalin-fixed neutrophils (d); expected C-ANCA revealing an atypical pattern by visual score on ethanol-fixed neutrophils and erroneously scored P-ANCA by AKLIDES (e), but consistent C-ANCA on formalin-fixed neutrophils (f); P-ANCA on ethanol-fixed neutrophils erroneously scored C-ANCA by AKLIDES (g) and consistent negative on formalin-fixed neutrophils (h); intermediate positive (1/320) homogenous ANA reported as P-ANCA on ethanol-fixed neutrophils by AKLIDES (i), being consistently negative on formalin-fixed neutrophils (j); BPI-ANCA revealing a weak C-ANCA pattern by visual score on ethanol-fixed neutrophils (k), scored negative by AKLIDES on both ethanol- and formalin-fixed neutrophils (l).