Inhibition of leukocyte migration by an α 4 integrin antagonist in experimental colitis. Leukocytes were isolated from mesenteric lymph nodes (MLNs) of β-actin luciferase mice and injected into recipient mice with dextran sodium sulphate- (DSS-) induced colitis. The recipient mice received vehicle or α 4 antagonist, 1 hour pre cell transfer. Whole body and organs ex vivo (colon and small intestine) were imaged using an IVIS 100 charge-coupled device imaging system 4 hours following transfer. The pseudocolored images represent light intensity, where red is the strongest, and violet is the weakest signal. Inhibition is detected in the colon and in Peyer’s patches of the α 4 antagonist-treated mice.
