Gene Expression Profile Reveals Abnormalities of Multiple Signaling Pathways in Mesenchymal Stem Cell Derived from Patients with Systemic Lupus Erythematosus

Figure 4

mRNA and protein levels of cyclin D and cyclin E in BMMSCs from SLE patients. (a) Immunoblotting analysis of cyclin D and cyclin E. (b) qRT-PCR studies of the expression of cyclin D and cyclin E2 in BMMSCs from SLE and normal controls (𝑛=5, 𝑃>0.05). Results were shown as mean ± SEM, each performed with triplicate samples. (c) Quantity analysis showed low protein level of cyclin E in cells from SLE patients, **𝑃<0.01 by Student’s 𝑡-test. SLE: systemic lupus erythematosus, Nor: normal controls.

(a)

(b)

(c)