Table 2: Summary of rabbit immunogenicity studies with different EV71 vaccine candidates.

EV71 vaccine candidates Adjuvant formulated Total IgG titers Western blot Virus neutralization titer
VP1-43VP2-28rVP1rVP2rVP3EV71 (B4 E59)CVA16 (N5079)

2 μg of inactivated
 EV71 virion PBS 1/200 1/800 +++ +++ ++ 1/128 <1/8
 EV71 virion Alum 1/200 1/6400 +++ +++ ++ 1/6400 1/32

5 μg of inactivated
 EV71 virion Alum 1/200 1/6400 +++ +++ +++ 1/12800 1/64

Two to three rabbits per group were immunized 3 times with EV71 vaccine candidates alone or formulated with alum. The protocols for IgG titer determination, western blot analysis, and virus neutralization assay are described in Section 2.