Nucleotide-Binding Oligomerization Domain-1 and -2 Play No Role in Controlling Brucella abortus Infection in Mice

Figure 3

TNF-α production induced by B. abortus in macrophages, but not IL-12 and IL-6, requires NOD1, NOD2, and Rip2. Bone marrow from C57BL/6, NOD1^−/−, NOD2^−/−, and RIP2^−/− mice cells was differentiated in macrophages and stimulated with B. abortus S2308 (MOI 100 : 1) or E. coli LPS (1 μg/mL). Supernatants was harvested for measuring IL-12 (a), TNF-𝛼 (b), and IL-6 (c) after 24 hrs by ELISA. Statistically significant difference in relation to non-stimulated cells is denoted with # and in relation to C57BL/6 mice is denoted with an asterisk (∗). The significance of differences was compared by ANOVA followed by Tukey’s test (𝑃<0.01).

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