The 19 kDa Mycobacterium tuberculosis Lipoprotein (LpqH) Induces Macrophage Apoptosis through Extrinsic and Intrinsic Pathways: A Role for the Mitochondrial Apoptosis-Inducing Factor
Figure 2
LpqH triggers a TLR2 cell death program. To determine a possible role of TLR2 in MO apoptosis, inhibition assays were performed using a specific mAb known by its ability to block TLR2 activation; cells were preincubated with 20 μg antibody for 1 h and then with 5 μg LpqH to induce apoptosis. A similar procedure was used to analyze the participation of TLR4. Apoptosis was analyzed with a nucleosome ELISA kit. Values are given as the mean ± SE of four independent experiments. Statistically significant differences are indicated; .
