The 19 kDa Mycobacterium tuberculosis Lipoprotein (LpqH) Induces Macrophage Apoptosis through Extrinsic and Intrinsic Pathways: A Role for the Mitochondrial Apoptosis-Inducing Factor
Figure 3
In the LpqH-induced apoptosis participate Fas/FasL. (a) MOs were incubated for 1 h with 5 μg LpqH and the expression of Fas and FasL was quantitated by a cell surface ELISA method using specific mAb. The relative fold increase over untreated cells (set to 1) is shown as the mean ± SE of four independent experiments; statistically significant differences are indicated; . (b) Inhibition assays to establish the role of Fas and FasL were carried out. MOs were preincubated with neutralizing mAb to Fas and FasL. Thereafter, without rinsing, cells were incubated with 5 μg LpqH for 1 h. The mean ± SE apoptosis level of 3 independent experiments is indicated. ; .
