125643.fig.006a
(a)
125643.fig.006b
(b)
Figure 6: DXM inhibited human MDDC activation. Immature MDDCs were treated with LPS (100 ng/mL) + IFN-γ (10 ng/mL), LPS (100 ng/mL) + IFN-γ (10 ng/mL) + DXM (25, 50 μM) for 18 h. The control group was treated with PBS alone. (a) Supernatants were collected 18 h later (TNF, 6 h), and TNF-alpha, IL-6, and IL-12 production was measured by ELISA. Data are presented as the mean ± SD of samples from three wells. Significant differences between DXM-treated and untreated LPS + IFN-γ-activated DCs are shown with asterisks ( ). (b) The expression of CD80, CD83, and HLA was determined by flow cytometry. All data were gated on CD1a+ cells. The gray-filled area represents staining with an isotype-matched control Ab. The change of geometric mean fluorescence intensity in the LPS + IFN-γ or LPS + IFN-γ + DXM samples is indicated. All data are representative of five independent experiments with cells from individual donors.