p53/p21 Pathway Involved in Mediating Cellular Senescence of Bone Marrow-Derived Mesenchymal Stem Cells from Systemic Lupus Erythematosus Patients
Figure 2
The expression of p53/p21 and cell cycle-related molecules in BM-MSCs from SLE patients. Western blot was used to analyze p53/p21 and their relative proteins expressions. (a) The expression of p53 and p21 was significantly increased in BM-MSCs from SLE patients. (b) Quantification of p53 and p21 protein levels: the relative levels of protein expressions were normalized to GAPDH expression. Values are means ± SD of three experiments (*; #). (c) Western blot analysis and the expressions of Cyclin E, CDK2, and p-Rb in BM-MSCs from SLE patients were decreased. (d) Quantification of Cyclin E and CDK2 protein levels: the relative levels of protein expressions were normalized to GAPDH expression. Values are means ± SD of three experiments (*; #). (e) Quantification of p-Rb and Rb protein levels: the relative levels of protein expressions were normalized to GAPDH expression. Values are means ± SD of three experiments (*). GAPDH was used as the internal control. Total p53 and p21 were significantly increased in BM-MSCs from SLE patients, while the expressions of Cyclin E, CDK2, and p-Rb were significantly decreased in BM-MSCs of SLE patients when compared with those in normal control.
