Research Article

p53/p21 Pathway Involved in Mediating Cellular Senescence of Bone Marrow-Derived Mesenchymal Stem Cells from Systemic Lupus Erythematosus Patients

Figure 3

Analysis of the location and expression of p53 and p21 in the cytoplasmic and nuclear of BM-MSCs from SLE patients. ((a), (d)) Immunofluorescence staining to analyze the location of p53 and p21 in BM-MSCs. In the BM-MSCs from SLE patients, there was a clear increase in nuclears p53 and p21 expression. The scale bar is 25 mm. ((b), (e)) Western blot analysis of the cytoplasmic and nuclear p53 and p21 expressions. β-Actin was used as the internal control for the cytoplasmic proteins, whereas β-tubulin was used as the internal control for the nuclear proteins. ((c), (f)) Quantification the expressions of p53 and p21 in the nuclear and cytoplasmic. Compared with normal control, the location and expression of p53 and p21 in the nuclear of BM-MSCs were significantly higher in SLE patients. The relative levels of protein expressions were normalized to β-actin expression. Values are means ± SD of three experiments (* ).
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