p53/p21 Pathway Involved in Mediating Cellular Senescence of Bone Marrow-Derived Mesenchymal Stem Cells from Systemic Lupus Erythematosus Patients
Figure 4
Analysis of the location and expression of Cyclin E and CDK2 in the cytoplasmic and nuclear of BM-MSCs from SLE patients. ((a), (d)) The locations of Cyclin E and CDK2 in BM-MSCs were tested by immunofluorescence staining. We found that the expression of Cyclin E and CDK2 was notably decreased in nuclear of BM-MSCs from SLE patients, while there were notably higher expressions in nuclears in normal control. The scale bar is 25 mm. ((b), (e)) Western blot to analyze the expressions of Cyclin E and CDK2 in cytoplasmic and nuclear. ((c), (f)) Quantification the expressions of Cyclin E and CDK2 in the nuclear and cytoplasmic. Compared with normal control BM-MSCs, Cyclin E and CDK2 in the nuclear were significantly lower in SLE patients; the relative levels of protein expressions were normalized to β-actin expression. Values are means ± SD of three experiments (*).
