p53/p21 Pathway Involved in Mediating Cellular Senescence of Bone Marrow-Derived Mesenchymal Stem Cells from Systemic Lupus Erythematosus Patients
Figure 5
p21 siRNA decreased p21 expression in BM-MSCs. Cells were transfected with p21 siRNA for 24 hours. p21 expression was significantly decreased in BM-MSCs both in SLE patients and normal control by immunoblot analyses ((a)-(b)) and RT-PCR ((c)-(d)). The relative levels of protein expressions and gene expression of target mRNA were normalized to GAPDH expression. Values are means ± SD of three experiments (#).
