Potential Use of Bisphosphonates in Invasive Extramammary Paget’s Disease: An Immunohistochemical Investigation
Figure 3
Anti-CD163, anti-MMP-9, anti-B7H1, and anti-ARG1 antibody staining of noninvasive and invasive EMPD. Paraffin-embedded tissue samples from patients with non-invasive EMPD ((a), (c), (e), and (g)) and invasive EMPD ((b), (d), (f), and (h)) were deparaffinized and stained with the anti-CD163 Ab ((a) and (b)), anti-MMP-9Ab ((c) and (d)), anti-B7H1-Ab ((e) and (f)), or anti-ARG1 Ab ((g) and (h)). Sections were developed with liquid permanent red. (CD163: staining for macrophages, MMP-9 staining, and B7H1 for macrophages and endothelial cells) ((a)–(e), (g) (h): 200, (f): 400). Five representative fields of each section were selected from each group of EMPD. The number of CD163+ cells in invasive and noninvasive EMPD was counted using an ocular grid of 1 cm2 at a magnification of 400x and summarized. The data are expressed as the means SD of the numbers in each area. Stars: .
