Figure 3: Baicalin inhibits splenic Th17 cell expansion in vivo. Adjuvant-induced arthritis mice were injected intraperitoneally daily with 100 mg/kg Baicalin or phosphate-buffered saline (vehicle control) between postimmunization days 14 to 21. (a) Spleen index of control and adjuvant-induced arthritis mice with or without Baicalin treatment ( for each group). Spleen photographs are shown in the lower panels (b). Isolated splenocytes were stained and sorted by flow cytometry first for CD4+ T cells. IL-17+ T cells were analyzed in the CD4+ gate (left). The percentage of dual positive CD4+IL-17+ cells among the gated CD4+ T cell fraction in each treatment group is shown on the right ( for each group). (c) IL-17 gene expression in joint tissues was analyzed by RT-PCR. These experiments were performed three times with similar results. (d) IL-17 gene expression in joint tissues was analyzed by real-time RT-PCR ( for each group).