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Figure 4: Baicalin inhibits IL-17-mediated inflammation reactions in vitro. (a) Synoviocytes (10,000 cells/well) were plated in 12-well tissue culture plates, allowed to adhere, and were then stimulated with 50 ng/mL IL-17 with or without 20 μM Baicalin for 24 hours. Following IL-17/Baicalin stimulation, isolated splenocytes (50,000 cells/well) were added to the synoviocyte monolayers for another 24 hours. After washing twice, splenocyte adherence to synoviocytes was enumerated by microscopy at 200x magnification. Arrows indicate adhesive splenocytes (left). The number of adherent splenocytes is shown on the right. These experiments were performed three times with similar results. In mRNA expression studies, synoviocytes were cultured with 50 ng/mL IL-17 in the presence or absence 20 μM Baicalin for 24 hours, and gene expression levels of ICAM-1 (b), VCAM-1 (c), IL-6 (d), and TNF-α (e) were examined by real-time RT-PCR. These experiments were performed three times with similar results.