Combinations of TLR Ligands: A Promising Approach in Cancer Immunotherapy
Figure 1
Direct cytotoxicity of TLR ligands towards CRC cell lines. Tumor cells were treated with increasing concentrations of the TLR ligands (a) R848 and (b) Taxol for 72 h. Cell viability was assessed by standard MTS assay. Results for the HROC60 cell line reached statistical significance ( versus control). (c) HROC60 cells were treated with TLR ligands for 24, 48, and 72 h with different concentrations (0.01–10 μM). (d) HROC60 cells were treated with Taxol for 24, 48, and 72 h with different concentrations (0.01–10 μM). Antitumoral effects were determined by a flow cytometric assay. Given results reached significance ( versus control). (e) The effect of increasing Taxol concentrations on CRC cell lines was assessed after 72 h incubation by flow cytometry. Results for HROC60 cells reached statistical significance at all concentrations; significant growth inhibition of HROC40 and HROC69 cells was obtained at 10 μM and 1 μM ( versus control). Untreated cells without TLR ligand were set as 1 and all data are given as X-fold increase. Experiments were performed in duplicate and repeated at least three times. Values are given as mean ± SD; versus control; t-test.
