The Leech Nervous System: A Valuable Model to Study the Microglia Involvement in Regenerative Processes
Figure 2
Observation of microglial cell accumulation from isolated and crushed segment of nerve cord maintained in tissue culture (see the diagram (c)). Microglial cell nuclei (white) were stained with Hoechst fluorescent dye to observe cell accumulation at the crush. (a) Six hours following a lesion of connectives, microglial cells progressively migrate toward the lesion site. (b) Later, 24 hours after the crush, microglial cells are mainly recruited to the lesion. This accumulation can be studied as much in vivo as in vitro.
