Dexamethasone Preconditioning Improves the Response of Collagen-Induced Arthritis to Treatment with Short-Term Lipopolysaccharide-Stimulated Collagen-Loaded Dendritic Cells
Figure 3
Cytokine production by CD4+ T cells from mice with active collagen-induced arthritis (CIA), treated with an inoculation of 4-hour lipopolysaccharide-(LPS-) stimulated dendritic cells (DCs) and dexamethasone (DXM) preconditioning. Mice received 2.0 mg/kg DXM from day 29 to day 34 after CIA induction (DXM group). Another group of mice were additionally injected at day 35 with 4-hour LPS-stimulated DCs loaded with type II collagen (CII) (DXM/4hLPS/CII/DCs group). The 4hLPS/CII/DCs group received 4-hour LPS-stimulated DCs loaded with CII, but without DXM preconditioning. The CIA control group corresponds to mice that did not receive any treatment. CD4+ T cells purified from mouse spleens at day 47 after CIA induction were stimulated with concanavalin A for 72 hours, and IL-10, IL-17, and IFN- levels in supernatants were quantified by ELISA. Bars represent the mean of three experiments.
