Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis
Figure 5
Effect of miR-155 on cytokines secretion of RA-FLS. (a) Increased level of miR-155 suppressed MMP-3 expression (, , 747.61 ± 72.19), while decreased level of miR-155 enhanced MMP-3 expression compared to NC in RA-FLS (, , 5263.57 ± 1260.16, 2605.23 ± 843.58, resp.). (b) Secretion of MMP-9 was not affected whether miR-155 expression was increased or decreased in RA-FLS compared to NC (, , 1712.28 ± 106.41, 1671.08 ± 452.25, 1535.36 ± 192.23, resp.). (c) Secretion of TGF-β was not affected whether with increased or decreased expression of miR-155 in RA-FLS compared to NC (, , 988.22 ± 113.55, 945.88 ± 94.56, 943.86 ± 258.72, resp.). The cells were transfected with 100 nM miR-155 mimic or miR-155 inhibitor for 48 h under 20 ng/mL TNF-α stimulation. Each experiment was in three replicates.
