Immunomodulatory Effect of Bisphosphonate Risedronate Sodium on CD163+ Arginase 1+ M2 Macrophages: The Development of a Possible Supportive Therapy for Angiosarcoma
Figure 1
Paraffin-embedded tissue samples from patients with recurrent AS after being treated with DTX monotherapy were deparaffinized and stained as follows: anti-CD163 Ab (a) and anti-MMP9 Ab (b). Sections were developed with liquid permanent red (A) and with 3,3′-diaminobenzidine tetrahydrochloride. M2 macrophages were induced from PBMC from healthy donors as described in Material and Methods section. M2 macrophages induced from PBMC from healthy donors were cultured for 5 days, treated with human IL-4 at day 5, and stimulated with DTX and/or RS. These cells were harvested from the culture and their expression of M2 macrophage-associated molecules (CD163, Arg1) was analyzed by flow cytometry.
