Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages
Figure 3
Effect of propofol pretreatment on LPS-induced phosphorylation of nuclear NF-κB and Akt. (a), (c) The cells were pretreated with dimethyl sulfoxide (DMSO) or propofol for 40 min and then stimulated with LPS for 1 h. Nuclear NF-κB and Akt activation, indicated by phosphorylation, was analyzed by Western blotting on whole cell lysates. (b), (d) The levels of phosphorylated NF-κB (p-NF-κB) and phosphorylated Akt (p-Akt) were quantified by measuring band intensities and represented as fold change over total NF-κB and Akt. Each value represents the means ± SD for . # and * indicate statistically significant differences () between propofol and LPS treated and LPS only treated groups, respectively.
