Propofol Reduces Lipopolysaccharide-Induced, NADPH Oxidase (NOX2) Mediated TNF-α and IL-6 Production in Macrophages
Figure 4
Effect of NF-κB Inhibitor on LPS-induced TNF-α and IL-6 expression. RAW264.7 macrophages were pretreated with dimethyl sulfoxide (DMSO) or 20 μM pyrrolidine dithiocarbamate (PDTC) for 1 h and stimulated with 100 ng/mL LPS for 8 h. The concentrations of TNF-α and IL-6 in culture supernatants were measured by ELISA. (c) RAW264.7 macrophages were pretreated with dimethyl sulfoxide (DMSO) or 20 μM PDTC for 1 h and then stimulated with LPS for 2 h. Steady state mRNA levels of TNF-α and IL-6 were examined by RT-PCR. (d) The levels of TNF-α and IL-6 mRNA were quantified by measuring band intensities and shown as fold increase relative to β-actin mRNA levels. Each value represents the means ± SD for . # and * indicate statistically significant differences () between propofol and LPS treated and LPS only treated groups, respectively.
