Possible Implication of Fcγ Receptor-Mediated Trogocytosis in Susceptibility to Systemic Autoimmune Disease

Figure 3

Comparison of endocytotic activities between neutrophils with and without display of FcγR-medicated trogocytosis. Peripheral blood polymorphonuclear cells (0.5 × 10⁶) and mononuclear cells (0.5 × 10⁶) were mixed and preincubated in 100 μL of the autologous serum for 20 min at 37°C. The cells were made to react with 0.1 μg of PECy5-labeled anti-CD8 Ab for 1 hour at room temperature. After washing with PBS, the cells were resuspended in 1 mL of RPMI-1640 medium with 10% FBS. Subsequently, the cells were made to react with 2 μL of yellow-green carboxylate-modified latex beads for 90 min at 37°C or with 100 μg of FITC-labeled OVA for 30 min at 37°C. After washing 3 times with PBS, the cells were re-suspended in 100 μL of PBS and then made to react with 0.1 μg of PE-labeled anti-CD15 Ab, followed by serving for FCM. Experiments were repeated at least 3 times. Similar results were reproduced.