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Clinical and Developmental Immunology
Volume 2013 (2013), Article ID 347213, 9 pages
http://dx.doi.org/10.1155/2013/347213
Research Article

Clinical Factors Influencing Phenotype of HCMV-Specific CD8+ T Cells and HCMV-Induced Interferon-Gamma Production after Allogeneic Stem Cells Transplantation

1Instituto Maimonides para la Investigacion Biomedica de Córdoba (IMIBIC), Reina Sofia University Hospital, 14004 Cordoba, Spain
2Department of Infectious Diseases and IMIBIC, Reina Sofia University Hospital, Avenida Menéndez Pidal s/n, 14004 Cordoba, Spain
3Spanish Network for Research in Infectious Diseases (REIPI), IMIBIC, Reina Sofia University Hospital, Avenida Menéndez Pidal s/n, 14004 Cordoba, Spain
4Department of Haematology and IMIBIC, Reina Sofia University Hospital, Avenida Menéndez Pidal s/n, 14004 Cordoba, Spain
5Department of Immunology and IMIBIC, Reina Sofia University Hospital, Avenida Menéndez Pidal s/n, 14004 Cordoba, Spain

Received 25 June 2012; Revised 29 December 2012; Accepted 2 January 2013

Academic Editor: Hans Hellmut Hirsch

Copyright © 2013 Inmaculada Gayoso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Human cytomegalovirus (HCMV) infection causes significant morbidity and mortality after hematopoietic stem cell transplantation (HSCT). In this work, we characterized the phenotype and interferon-gamma (INF- ) production of HCMV-specific T cells using QuantiFERON-HCMV assay in 26 patients 6 months after HSCT. We analysed whether these two parameters were associated with clinical variables. Our results showed that the patients receiving stem cells from donors ≥40 years old were 12 times more likely to have HCMV-specific CD8+ T cells with “differentiated phenotype” (CD45RA+CCR7+ ≤6.7% and CD28+ ≤30%) than patients grafted from donors <40 years old ( ; ). In addition, a detectable IFN- production in response to HCMV peptides (cutoff 0.2 IU/mL IFN- ; “reactive” QuantiFERON-HCMV test) was statistically associated with HCMV replication after transplantation ( ; ), recipients ≥40 versus <40 years old ( ; ), and the use of peripheral blood versus bone marrow as stem cell source ( ; ). In conclusion, donor age is the only factor significantly associated with the presence of the “differentiated phenotype” in HCMV-specific CD8+ T cells, whereas HCMV replication after transplantation, recipient age, and stem cell source are the factors associated with the production of IFN- in response to HCMV epitopes.