TSE Diagnostics: Recent Advances in Immunoassaying Prions

Table 2

Summary of the methods for detection of PrP^Sc in blood.

Reference	PrP source	PK	Denaturation	Antibodies	Detection method	Sensitivity*
[44]	Scrapie mice blood and CWD deer and elk blood	−	−	n.r.	FACCT	n.r.
[38]	CJD human blood	+	−	C: 6H4; D: 3F4-biotin; Streptavidin-Eu	DELFIA	50 ul recPrP/mL PK-digested plasma; 10 pg recPrP/well
[45]	Scrapie sheep blood and CWD white-tailed deer blood	−	1% SDS	C: 11F12; D: 5D6-biotin	SOFIA	n.r.
[46]	Healthy human blood spiked with vCJD brain	Thermolysin	4 M GdnHCl	C: ICSM 10; D: ICSM 35-biotin	Sandwich ELISA	2.8 pg PrP^Sc/well; 150000-fold dilution (10^5,17)
[47]	vCJD human blood and healthy human blood spiked with vCJD brain	−	Heat	D: ICSM 18-biotin	ELISA	10^10-fold dilution of vCJD brain homogenate in whole blood

∗We report the sensitivity as provided by the authors because of the lack of sufficient data for converting the results to the united form.
rHuPrP: recombinant human prion protein, rMoPrP: recombinant mouse prion protein, rOvPrP: recombinant ovine prion protein, rBoPrP: recombinant bovine prion protein, rDePrP: recombinant deer prion protein, rHaPrP: recombinant hamster prion protein.
ICSM is not an acronym but a name of two anti-prion antibodies (ICSM 35, ICSM 18).