Mesoporous Silicon Microparticles Enhance MHC Class I Cross-Antigen Presentation by Human Dendritic Cells

Figure 4

Dendritic cells maturation by MSMPs. (a) Representative histograms of dendritic cells maturation determined by quantifying the presence of the following activation markers: HLA-class I, CD80, CD86, and HLA-class II (DR) on their cell surface by flow cytometry. Isotype: irrelevant antibody; (−): MDDCs alone; CEFpp: MDDCs exposed to antigenic peptide (CEFpp) alone; MSMPs−: MDDCs with microparticles without CEFpp; MSMPs+: MDDCs with microparticles loaded with CEFpp; LPS: lipopolysaccharide. (b) Relative mean fluorescence intensity. Mean ± SD (𝑛=4). *𝑃<0.05; **𝑃<0.03; ***𝑃<0.01.

(a)

(b)