Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines
Figure 1
(a) Alignment of amino acid sequences of the wild type (Pru p 3) and mutant forms (Pru p 3.01, Pru p 3.02,and Pru p 3.03). The residues shaded in black correspond to the IgE epitope in Pru p 3 or the modified residues in Pru p 3.01, Pru p 3.02, and Pru p 3.03. The molecular weight (MW) obtained by mass spectrometry is indicated. (b) The recombinant proteins (3 g) were separated by SDS-PAGE and stained with Coomassie Blue (Coomassie) or immunodetected with anti-Pru p 3 antibody (anti-LTP). (c) Far-UV (190–260 nm) CD spectrum of purified proteins.
