Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines
Figure 3
(a) Specific IgG1 and IgG4 to Pru p 3 forms (wild type, Pru p 3.01, Pru p 3.02, and Pru p 3.03), using serum pool from peach-allergic patients (1 : 10 dilution). Blocking solution without solid phase was used as a negative control, and optical density (OD) values > 0.086 units (; mean + 3x SD = 0.05 + 3 × 0.012 OD units) were considered positive. All tests were performed in triplicate. (b) Reduction of IgG1 and IgG4 binding capacity (%) of the mutants in comparison with Pru p 3 was calculated by taking the value of Pru p 3 (5 μg/mL) to represent 100% in each serum sample. *The number of the patients corresponds to that in Table 1. −, negative value, represents those patients with negative IgG4 for Pru p 3.
