The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics

<table>Subcellular localization of the PAFR in resting neutrophils. Neutrophil subcellular organelles from disintegrated cells were fractionated on a three-layer Percoll gradient. Upper panel. The distribution of gelatinase (marker for the <i>β</i>-fractions) visualized through immunoblotting. Middle panel: localization of azurophil granules (<i>α</i>-fraction; marker MPO) specific granules (<i>β</i>1-fraction; markers NGAL) and plasma membrane/secretory vesicles (<i>γ</i>-fraction; ALP). The markers (MPO = ▲; NGAL = ●; ALP = ■) are expressed in arbitrary units. Lower panel: proteins from selected fractions were separated by SDS-PAGE, and the localization of PAFR was determined by immunoblotting with a specific antibody against PAFR. </table>

Journal of Immunology Research

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Figure 1

Figure 1: The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics 

Figure 1 | The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics 