The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 4
PAF triggers a rapid and WEB2086 sensitive release of superoxide anions from human neutrophils. Human neutrophils (105 cells) were incubated at 37°C for 5 min in measuring vials containing isoluminol and HRP in the absence (control, solid line in the main figure) or presence of the PAFR antagonist WEB2086 (dashed line in the main figure). The cells were then activated with PAF (100 nM, added at arrow), and the release of superoxide anions was recorded continuously. Inset: the kinetics of superoxide production induced by PAF (100 nM; solid line) was compared to that induced by fMLF (100 nM; dashed line), and the times required to reach the respective peak of the response are given. Data are derived from one representative experiment out of at least five. Abscissa: time of study (min); ordinate, superoxide production expressed as counts per minute 106 (Mcpm; main figure) or normalized to compare the time courses (inset).
