The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 5
The PAF-induced neutrophil response is primed by TNF-α and inhibitors of actin polymerization. Human neutrophils were incubated at 37°C for 20 min with the priming agents TNF-α (10 ng/mL; black bars) or 5 min with either CytB (5 μg/mL; grey bars) or latrunculin A (50 ng/mL; white bars). Control cells were incubated at the same conditions but in the absence of any priming agents. The cells were then activated with fMLF (100 nM) or PAF (100 nM), and the release of superoxide was recorded continuously. Data are expressed as fold increase of the peak values of the response from primed cells compared to nonprimed control (mean ± SEM; ). The dashed line in the graph denotes the value expected with a nonactive priming agent.
