The Subcellular Localization of the Receptor for Platelet-Activating Factor in Neutrophils Affects Signaling and Activation Characteristics
Figure 8
Effect of the PI3-kinase inhibitor wortmannin on the PAF and fMLF-induced release of superoxide anions. Human neutrophils (105 cells) were incubated without (control), or with wortmannin (10 nM or 100 nM) for 10 min at 37°C. The cells were then stimulated with PAF (100 nM) or fMLF (100 nM), and the release of superoxidewas recorded continuously. Effects of wortmannin expressed in % of superoxide production (peak values compared) obtained without any additive (mean ± SEM; ).
