Role of Toll-Like Receptor 4 on Lupus Lung Injury and Atherosclerosis in LPS-Challenge ApoE^−/− Mice

Figure 4

Double immunofluorescence labeling of TLR4 and F4/80⁺ macrophages in lung and aortic sinus plaque. Distribution of TLR4 (green) in macrophages detected by F4/80 (red) from lung ((a)–(f)) and aortic sinus plaques ((g)–(i)). Nuclei were stained with DAPI (blue). The arrow indicated macrophages located at the deeper layers of the plaque after LPS challenge (200x).

(a) Lung in ApoE^−/− NS group

(b) Lung in ApoE^−/− LPS group

(c) Lung in ApoE^−/− LPS + TAK-242 group

(d) Lung in wild-type NS group

(e) Lung in wild-type LPS group

(f) Lung in wild-type LPS + TAK-242 group

(g) Plaque in ApoE^−/− NS group

(h) Plaque in ApoE^−/− LPS group

(i) Plaque in ApoE^−/− LPS + TAK-242 group