Effects of Muscarinic Acetylcholine 3 Receptor208-227 Peptide Immunization on Autoimmune Response in Nonobese Diabetic Mice
Figure 4
Reductions in the levels of IL-17 and IFN-γ in the lacrimal glands following immunization with the M3R208–227 peptide. To further observe whether the concentrations of cytokines decreased in the lacrimal glands, we also examined the production of IFN-γ, IL-4, and IL-17 in the lacrimal glands of NOD/LtJ mice by immunofluorescence staining. Whole lacrimal glands from NOD/LtJ mice were removed, fixed, embedded, and incubated with primary antibodies, followed by a secondary goat anti-rat IgG antibody. The results of immunofluorescence staining demonstrated that the production of IL-17 and IFN-γ decreased in the lacrimal glands of NOD/LtJ mice immunized with the M3R208–227 peptide. In contrast, the production of IL-4 was not observed in the lacrimal glands of NOD/LtJ mice in all groups.
