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Clinical and Developmental Immunology
Volume 2013 (2013), Article ID 506807, 9 pages
http://dx.doi.org/10.1155/2013/506807
Research Article

O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin

1Unidad de Investigación, Instituto de Oftalmología Fundación Conde de Valenciana, 06800 México, DF, Mexico
2Laboratorio de Inmunología, Departamento de Bioquímica, Facultad de Medicina, (UNAM), P.O. Box 70159, 04510 México, DF, Mexico
3Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, IPN, 11340 México, DF, Mexico
4Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, 4502 México, DF, Mexico

Received 18 June 2013; Accepted 4 August 2013

Academic Editor: Lenin Pavon

Copyright © 2013 María C. Jiménez-Martínez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

O-glycosidically-linked glycans have been involved in development, maturation, homing, and immune regulation in T cells. Previous reports indicate that Amaranthus leucocarpus lectin (ALL), specific for glycans containing galactose-N-acetylgalactosamine and N-acetylgalactosamine, recognizes human naïve CD27+CD25+CD4+ T cells. Our aim was to evaluate the phenotype of CD4+ T cells recognized by ALL in peripheral blood mononuclear cells obtained from healthy volunteers. CD4+ T cells were isolated by negative selection using magnetic beads-labeled monoclonal antibodies; the expression of T regulatory cell phenotypic markers was assessed on ALL-recognized cells. In addition, IL-4, IL-10, IFN- , and TGF- intracellular production in cells was also evaluated. The analyses of phenotypic markers and intracellular cytokines were performed through flow cytometry. ALL-recognized CD4+ T cells were mainly CD45RA+, CCR7+ cells. Although % CD25+Foxp3+ cells were positive to ALL, only % of cells corresponded to CD25+Foxp3 cells. Intracellular cytokines in freshly obtained CD4+ T cells exhibited 8% of IL-4, 15% of IL-10, 2% of IFN- , and 15% of TGF- , whereas CD4+ T cells depicted 1% of IL-4, 2% of IL-10, 1% of IFN- , and 6% of TGF- . Our results show that galactose-N-acetylgalactosamine and N-galactosamine-bearing CD4+ T cells expressed phenotypic markers of NnTreg cells.