O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin

Figure 1

ALL recognizes purified CD4⁺ T cells. (a) Freshly purified CD4⁺ T cells were stained with CyChrome-labeled streptavidin alone (thick line) after incubation with biotin-labeled ALL (thin line). The bar denotes percentage of purified ALL ⁺CD4⁺ T cells. (b) GalNAc inhibited Amaranthus leucocarpus lectin (ALL) binding sites for CD4⁺T cells. Gray lines denote the staining of cells treated only with CyChrome-streptavidin; thin line indicates the florescence level of ALL ⁺ cells; thick line denotes fluorescence of ALL ⁺ cells when they were treated with GalNAc.

(a)

(b)