O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin

Figure 3

CD25 and Foxp3 frequency on ALL ⁻ and ALL ⁺CD4⁺ T cells. To analyze cell surface marker staining, a gate was drawn around the lymphocyte population based on their physical characteristics (forward) and positive fluorescence to CD25; a second gate was drawn based on positive or negative fluorescence to Foxp3. CD25⁺ cells positive or negative to Foxp3 were analyzed by binding to ALL.