O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin

Figure 4

Frequency of intracellular cytokine positive cells on ALL ⁻ and ALL ⁺ gated cells. Freshly purified CD4⁺ T cells were incubated with ALL-Biotin followed by CyChrome-streptavidin incubation and stained with monoclonal antibodies against IL-4, IL-10, IFN-γ, or TGF-β. Cells were analyzed by flow cytometry and gated on ALL ⁻ or ALL ⁺ cells. Percentages of cells positive to intracellular cytokines are shown in histograms.