O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin

Figure 5

Polyclonal stimulation induced high expression of Foxp3 and TGF-β in ALL ⁺ T helper cells. CD4⁺ T cells were stimulated with Concanavalin-A (2 μg/mL). After 48 h, cells were stained with biotin-ALL followed by CyChrome-streptavidin, and stained with monoclonal antibodies against CD25 and Foxp3 or TGF-β. (a) Dot plots of nonstimulated cells (RPMI alone) showing frequency of CD25⁺Foxp3⁺ in ALL ⁻ and ALL ⁺ T helper cells. After polyclonal stimulation An increased frequency of both (b) CD25⁺Foxp3⁺ cells and (c) TGF-β ⁺ cells (thick line) in ALL ⁺ T helper cells was observed. (Thin line corresponds to isotype control).

(a)

(b)

(c)