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Clinical and Developmental Immunology
Volume 2013 (2013), Article ID 524204, 4 pages
http://dx.doi.org/10.1155/2013/524204
Research Article

Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients

1Department of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, China
2Department of Rheumatology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, China

Received 1 May 2013; Accepted 5 September 2013

Academic Editor: Jianying Zhang

Copyright © 2013 Qiao Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP). Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay. Results. miR-124-a1 and miR-124-a2 promoter methylation were both seen in 71.4% of RA samples compared to 16.7% of OA samples. miR-124-a3 promoter methylation was seen in 57.1% of RA samples and 0% of OA samples. All the three loci were unmethylated in 3 healthy controls. Conclusion. The methylation status of miR-124a seen in this study concurs with that reported in tumor cells, indicating epigenetic dysregulation constituents, a mechanism in the development of rheumatoid arthritis.