Development of a Recombinant Cell-Based Indirect Immunofluorescence Assay for the Determination of Autoantibodies against Soluble Liver Antigen in Autoimmune Hepatitis

Figure 3

Immunofluorescence staining patterns of HEK293 expressing soluble liver antigen. HEK293 expressing soluble liver antigen (SLA) and wild-type HEK293 were incubated with either 1 : 100 diluted human serum or 1 : 100 diluted anti-SLA rabbit serum and bound antibodies were visualized with either anti-human IgG FITC (green) or anti-rabbit IgG Cy3 (red) conjugates. Nuclear DNA was stained with TO-PRO-3 (blue). (a, c, e) HEK293-SLA; (b, d, f) untransfected HEK293; (a & b) representative anti-SLA positive serum from a patient with autoimmune hepatitis; (c & d) representative anti-SLA negative serum from a healthy blood donor; (e & f) anti-SLA rabbit serum.