Overexpression of HIF-2α, TWIST, and CXCR4 Is Associated with Lymph Node Metastasis in Papillary Thyroid Carcinoma
Table 2
Immunohistochemical analysis of HIF-2, TWIST, and CXCR4 expression in 129 PTCs, 61 nodular hyperplasia, and 118 normal thyroid tissue specimens according to the scoring system.
Score
HIF-2α
TWIST
CXCR4
Normal thyroid tissue (%)
Nodular hyperplasia (%)
PTC (%)
Normal thyroid tissue (%)
Nodular hyperplasia (%)
PTC (%)
Normal thyroid tissue (%)
Nodular hyperplasia (%)
PTC (%)
0
Negative
94 (79.7)
38 (62.3)
4 (3.1)
97 (82.2)
40 (65.6)
3 (2.3)
91 (77.1)
36 (59.0)
3 (2.3)
+
1
21 (17.8)
18 (29.5)
7 (5.4)
19 (16.1)
16 (26.2)
12 (9.3)
23 (19.5)
19 (31.1)
8 (6.2)
2
3 (2.5)
4 (6.6)
16 (12.4)
2 (1.7)
5 (8.2)
17 (13.2)
4 (3.4)
4 (6.6)
13 (10.1)
3
0 (0)
1 (1.6)
18 (14.0)
0 (0)
0 (0)
19 (14.7)
0 (0)
2 (3.3)
16 (12.4)
4
0 (0)
0 (0)
20 (15.5)
0 (0)
0 (0)
22 (17.1)
0 (0)
0 (0)
18 (13.9)
++
6
0 (0)
0 (0)
16 (12.4)
0 (0)
0 (0)
19 (14.7)
0 (0)
0 (0)
19 (14.7)
8
0 (0)
0 (0)
23 (17.8)
0 (0)
0 (0)
17 (13.2)
0 (0)
0 (0)
17 (13.2)
+++
9
0 (0)
0 (0)
16 (12.4)
0 (0)
0 (0)
17 (13.2)
0 (0)
0 (0)
20 (15.5)
12
0 (0)
0 (0)
9 (7.0)
0 (0)
0 (0)
3 (2.3)
0 (0)
0 (0)
15 (11.6)
The immunohistochemical scores in PTCs, nodular hyperplasia, and normal thyroid tissue specimens were determined as the multiplication of proportion score and intensity score.