Expression and Function of the Homeostatic Molecule Del-1 in Endothelial Cells and the Periodontal Tissue
Figure 1
Expression of endothelial adhesion molecules in Del-1 deficiency. (a) Gingiva dissected from 16-week-old C57BL/6 (WT) and mice were processed for quantitative real-time PCR analysis of mRNA expression of the indicated genes; results were normalized to those of GAPDH mRNA and expressed as fold change in transcript levels relative to WT, the average value of which was taken as 1. (b) Sagittal sections of interdental gingiva from 16-week-old C57BL/6 WT or mice were stained for ICAM-1, VCAM-1, or E-selectin and CD31, as indicated. Shown are representative overlays of differential interference contrast and fluorescent confocal images, with colocalization demonstrated in merged images (scale bar, 50 μm). (c) The fluorescence intensities of the images shown here and of additional representative images from independent mice (5 per group) were quantified using ImageJ analysis. In (a) and (c), data are means ± SD ( mice per group). No statistically significant differences were detected between and WT littermate controls.
