Research Article

Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression

Figure 4

(a) Regulation of BKV agnoprotein expression in the stably transfected UTA-6 clone 2C9. The panel shows a time course experiment using different concentrations of tetracycline. Blue: DNA stained with Hoechst 3342. Red: agnoprotein detected by indirect immunofluorescence. (b) HLA-ABC and -DR surface expression in UTA-6 clonal cells with Tet-off inducible agnoprotein expression. HLA-ABC and -DR surface expression was detected by flow cytometry in the UTA-6 clone 1C5 (first row) and 2C9 (second row) and cultured for 48 hours with different concentration of IFNγ and for 24 h in the presence or absence ( ) of Tet. MFI: mean fluorescence intensity.
626823.fig.004a
(a)
626823.fig.004b
(b)